Sep 14, 2006 · Plant organs (approximately 1 g) were pulverized to a fine powder in a mortar and pestle in the presence of liquid N 2. Powder was resuspended directly in the mortar with 15 ml of homogenization media [50% phenol, 0.45 M sucrose, 5 m M ethylenediaminetetraacetic acid, 0.2% (v/v) 2‐mercaptoethanol, 50 m M Tris–HCl, pH 8.8] with continued ...
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