Oct 01, 2004 · Each muscle was frozen in liquid nitrogen and ground to a fine powder. The material was resuspended in 1 ml of cell lysis buffer [25 mM Tris-phosphate, pH 7.8, 2 mM DTT, 2 mM 2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (Sigma), 10% glycerol, and 1% Triton X-100], and processed as described for cell lysates. Ten microliters of undiluted ...
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